methodology of d experiment...~

what's the most thing we do in MIB

mostly ktowang wat inoculation n extraction plasmid DNA..the DNA given come in bulk (approx 200 sumtin)..at 1st kami xtaw sample ape yg sprvsor bg ni..tp klw nk taw, leh type name sample tu kat ncbi..

huhuhu..tp xsempat lg nk cari..cz asal bkak intrnet jek, niat lari drpd yg asal..pttnye jari ni taip ncbi, tp tertaip fs, blog, fb, n mcm2 lg la huahuahua..tp senior ktowang gtaw tu sample kacang tp xpasti part mane..so..ni la task ktowang..wat mase ni..the next task will be given after d current task is done..


LETS BEGIN THE OPERATION~~~!!!
trademark yg pastinye menjadi bahan ketawa bdk2 MIB..
ko ni naz..xhbs2 dgn trademark tu..xde trademark lain ke? -xdek!-


inoculation

proses di bwh : utk prepare glycerol stock for future use...

seblah kanan skali : universal tube yg da ade growth of cells
kiri atas : propylene tube
tgh : flame
kiri skali : glycerol 50%
on my left hand : microcentrifuge tube
right hand : micropipette



pipetting kanamycin n sample into d tube



Broth containing the grown cells then filled into new propylene tube


extraction

extracted DNA



electrophoration

setelah itu, electrophoresis kan kan kan..

dna inserted into well of agarose gel..ni kami guna load 200 sample..tapi ktowang wat 60-90 je..sakit pinggang n sendi2 urat segale pas wat mende nih..last, result is taken..nampakla sgale band2 dna tu..

pasni nk wat transformation..peminat semua tggu ye huahuahua..